Articles (22)

Laboratory Safety Manual - Chapter 00: Introduction

This manual is a safety reference document for laboratory personnel at the University of North Carolina at Chapel Hill. The University’s Department of Environment, Health and Safety prepared this manual, followed by review and approval from both the University’s Laboratory and Chemical Safety Committee (LCSC) and the University Safety and Security Committee (USSC).

Laboratory Safety Manual - Chapter 01: Laboratory Safety at the University of North Carolina at Chapel Hill

This chapter outlines some of the basic regulations that govern laboratory safety, services offered by EHS, how to report injuries and incidents, laboratory self-inspections, and how to respond to fires and chemical spills.

Laboratory Safety Manual - Chapter 02: Laboratory Safety Plan

This chapter provides detailed instructions for completing and updating the various schedules of a Laboratory Safety Plan. The Laboratory Safety Plan is a required document that outlines specific conditions, hazards, and controls in your laboratory spaces.

Laboratory Safety Manual - Chapter 03: General Safety Principles and Practices

This chapter lists and describes several major categories of hazardous materials and/or hazardous operations that you could work with in your lab. For each category, the chapter includes recommended safe work practices and regulatory requirements (if applicable).

Laboratory Safety Manual - Chapter 04: Proper Storage of Chemicals in Laboratories

This chapter instructs you how to interpret the labels on chemical containers, and how to safely store chemicals in the laboratory in a way that minimizes incompatible chemical reactions, spillage, breaking, or waste due to expiration.

Laboratory Safety Manual - Chapter 05: Protective Clothing and Equipment

This chapter describes the various types of protective equipment and clothing that can protect you while working in the lab. Details for safe use, care, and acquisition are given for eye/face protection, gloves, lab apparel, foot protection, and respiratory protection.

Laboratory Safety Manual - Chapter 06: Safe Handling of Chemicals

This chapter discusses the major routes of exposure to chemical substances during laboratory work, and several safe handling practices that can minimize your risk while working with chemical substances. The last section lists practices for the safe use of hydrofluoric acid.

Laboratory Safety Manual - Chapter 07: Highly Toxic Chemicals and Select Carcinogens

This chapter supplements previous chapters by giving specific extra precautions, postings, training, and protective equipment necessary when working with substances that are highly toxic and/or select carcinogens. The appendices at the end of the chapter are a thorough (but not exhaustive) list of substances that might be present in your lab that are highly toxic and/or carcinogenic.

Laboratory Safety Manual - Chapter 08: Reproductive Hazards

This chapter supplements previous chapters by giving specific extra precautions, postings, training, and protective equipment necessary when working with reproductive hazards. These include chemical, biological, or radiological substances that can affect the developing fetus, or the reproductive health of the male or female parents. This chapter also outlines the UNC conceptus protection policy for laboratory workers who are pregnant or contemplating pregnancy.

Laboratory Safety Manual - Chapter 09: Controlled Substances

This chapter gives definitions and protocols for chemicals that are classified as controlled substances by the U.S. Drug Enforcement Administration. Controlled substances have special rules for acquisition, storage, security, inventory/recordkeeping, disposal, and importing or exporting, detailed in this chapter. The appendices include a current list of controlled substances and forms for inventory support and personnel screening.

Laboratory Safety Manual - Chapter 10: Fire Safety

This chapter outlines the properties of flammable liquids, solids, and gases, the proper storage and use of flammable substances, and the properties of fire extinguishers.

Laboratory Safety Manual - Chapter 11: Explosive and Reactive Chemical Hazards

This chapter provides resources that can help you prevent a laboratory accident due to mishandling explosive substances, or mixing incompatible reactive substances. This chapter details several specific examples of explosive and reactive hazards that are common in laboratories.

Laboratory Safety Manual - Chapter 12: Laboratory Waste Management Plan

The characterization, management storage and disposal of laboratory wastes (i.e., chemical waste including hazardous and non-hazardous solid waste, radioactive or mixed waste, biohazardous and medical waste, and universal waste) is regulated and requires strict compliance with regulatory obligations.

Laboratory Safety Manual - Chapter 13: Safe Handling of Peroxidizable Compounds

This chapter describes the hazards associated with peroxide formation in chemical compounds, methods to detect peroxides, safe handling, use, and storage of peroxidizable compounds, and how to remove peroxide contamination from chemicals.

Laboratory Safety Manual - Chapter 14: Safe Handling of Laboratory Animals

This chapter covers the hazards associated with laboratory animal handling, mandatory and recommended control practices, the institutional structures that UNC-Chapel Hill has in place to assure animal welfare, and requirements for using hazardous agents in laboratory animals.

Laboratory Safety Manual - Chapter 15: Safe Handling Of Biological Hazards

This chapter is an overview of the requirements for working with biological hazards. You can find more detailed information about working with biological hazards in the UNC Exposure Control Plan (Bloodborne Pathogens), the UNC Biological Safety Manual and on our website.

Laboratory Safety Manual - Chapter 16: Biological Safety Cabinets

This chapter describes the containment principles of biological safety cabinets (BSCs), the various classes and types of BSCs and their uses, how to select the correct type of BSC for your needs, how to get approval for installation of a BSC, and their installation and certification requirements.

Laboratory Safety Manual - Chapter 17: Laboratory Hoods

This chapter describes safe work practices when using laboratory hoods, answers frequently asked questions about this important engineering control equipment, and references the Hood and Laboratory Ventilation Policy at UNC-Chapel Hill.

Laboratory Safety Manual - Chapter 18: Safe Use of Nanomaterials

This chapter discusses the unique properties of nanomaterials, solid superatomic materials with at least one dimension in the range of one to 100 nanometers. Subsequent sections discuss the potential safety and health concerns from nanomaterials (based on cell culture and animal studies), the routes of exposure, and guidance on how to prevent exposures to nanomaterials.

Laboratory Safety Manual - Chapter 19: Research Laboratory Freezer Alarm Policy

To ensure that research laboratory freezer alarm systems (freezer alarms) are functional and compatible with existing monitoring equipment, this policy establishes guidelines for all university departments relating to the request for and installation and operation of freezer alarms. The existence of this policy is not a guarantee that the University will always be able to respond to freezer alarms or be able to restore temperate conditions quickly enough to prevent damage.

Laboratory Safety Manual - Chapter 20: Formaldehyde Exposure Control Policy

The Occupational Safety and Health Administration (OSHA) issued a standard (29 CFR 1910.1048) to ensure proper protection of all workers exposed to formaldehyde. The standard applies to all forms of formaldehyde including gas, aqueous solutions, solids, and materials that can release it.

Laboratory Safety Manual - Chapter 21: High School Students and Minors in Laboratories Policy

The concern of the University of North Carolina at Chapel Hill (“University”) for laboratory safety extends not only to employees but also to any persons visiting University laboratories, especially high school students and minors under the age of 18, who may potentially be exposed to hazardous materials. Laboratories are common sources of thermal dangers, compressed gases, electrical hazards, chemical, biological, and radioactive materials, lasers, and sharp objects.