Title
University of North Carolina at Chapel Hill Standard for Inhaled Anesthetics
Introduction
Purpose
The standards and procedures described below provide guidance to all researchers and animal handlers administering and handling inhaled anesthetics (e.g., isoflurane).
Scope
This Standard applies to all personnel engaged in the use of inhaled anesthetics in studies involving research animals.
The University of North Carolina at Chapel Hill ("UNC-Chapel Hill" or "University") Institutional Animal Care and Use Committee (IACUC) expects that anyone involved in animal work at the University will comply with this Standard. Requests for exceptions to this Standard must be reviewed and approved by the IACUC.
Standard
New researchers seeking certification in the use of an isoflurane vaporizer are first required to review IACUC, Environment, Health, & Safety (EHS) and Division of Comparative Medicine documents, complete an online EHS/DCM training Powerpoint, then complete the hands-on training. Details about vaporizer training and links to the required documents can be found on the DCM training page.
Please review the EHS Standard Operating Procedure for Isoflurane.
Vaporizer
Maintenance and Use
All anesthetic vaporizers must be serviced by a trained professional on an established schedule consistent with the manufacturer’s recommendations. A copy of the manufacturer’s recommendations should be available to the IACUC for review. At a minimum, vaporizers must be calibrated every two (2) years to ensure that the vaporizer is delivering the amount of anesthetic reflected by the setting on the vaporizer. All calibrations should be documented and readily available during IACUC laboratory inspections. A certification sticker applied to the vaporizer may serve as documentation and should indicate the name of the servicing agent and the date of service.
Additional indications of the need for vaporizer service includes the following:
- Discoloration (yellowish brown) in the "Fill" sight glass of a vaporizer
- Sticking valves or knobs
- Subjects not responding (as anticipated) to the level of anesthesia provided
- Cracked or damaged hoses
Users should assess the vaporizer for these changes prior to each use and contact the manufacturer for service if any abnormalities are found.
[Note: The National Institute for Occupational Safety and Health (NIOSH) (Health Care Workers Guidelines, Chapter 5) states that all anesthetic equipment must be regularly monitored for leakage, improper design, or defects. This includes the anesthesia machine as well as the vaporizer since anesthetic machines can develop improperly functioning components such as flutter valves, gaskets, and scavenging equipment.]
Induction Chamber
The induction chamber should be an appropriately-sized container for the species. Researchers should ensure the rubber seal remains pliable, the seal’s integrity prevents any leaks from the chamber, and the chamber has no cracks.
Opening the isoflurane induction chambers without the use of active scavenging can release vapors into the air. For this reason, researchers should flush the line with oxygen prior to opening the chamber. Ideally, induction chambers should be used in a hood, with a vacuum, or another system of active scavenging. If only passive scavenging is possible (e.g., charcoal canister), researchers should arrange for EHS to measure isoflurane levels during a typical procedure.
Vaporizer with Nosecone
When using a nosecone to administer isoflurane, it is important to ensure an adequate fit. To maintain an adequate anesthetic plane, an appropriately-sized nosecone should be used that covers the animal’s nose and mouth. Rubber diaphragms may be used in combination with a scavenging system to minimize the release of vapors into the air.
Drop Method
The ‘drop method’ is a rapid method to anesthetize rodents for very short procedures or to perform euthanasia via isoflurane overdose. Glass or plastic desiccators may be purchased from vendors or a customized version can be crafted. It is essential that the container be appropriately sized to prevent the need for large volumes of isoflurane or overcrowding of animals, which slows induction time. The container lid must be easily sanitizable and tight fitting to prevent potential isoflurane leaks. A protective layer (such as a perforated platform in a desiccator) is required inside the container to prevent direct contact of the animals with isoflurane-soaked materials. Contact with the anesthetic can cause skin irritation and potential overdose since isoflurane is absorbed through the skin.
Due to potential risk to human health, the use of the ‘drop method’ must occur in a fume hood, a ducted biosafety cabinet, or with a properly functioning active scavenging system. If this cannot occur, EHS must be contacted to measure the air quality. The investigator must receive EHS approval to use the ‘drop method’ on a bench top. Once bench top use is approved, the investigator must post a copy of the approval in the area.
For instructions on how to perform this procedure, please see the Standard Operating Procedure for Isoflurane Drop Method.
Exceptions
Requests for exceptions to this Standard must be reviewed and approved by the IACUC.
Definitions
NIOSH: National Institute for Occupational Safety and Health
Related Requirements
External Regulations and Consequences
University Policies, Standards, and Procedures
Contact Information
Policy Contact
- Name: Michael Chi
- Title: Associate Director, Office of Animal Care and Use
- Email: mchi@unc.edu
Subject: Animal Health
- Contact: DCM Vet Services
- Telephone: 919-962-5535
Subject: Office of Animal Care and Use
Important Dates
- Effective Date and title of Approver: 10/2018; UNC IACUC
- Revision and Review Dates, Change notes, title of Reviewer or Approver: 04/2018; UNC IACUC
Approved by: UNC IACUC