Use of Antibiotics in Animals Standard Operating Procedure

Summary

To ensure the safe handling and use of antibiotics in DCM facilities and research labs. The policy addresses risks associated with common antibiotics—some of which are known or suspected reproductive toxins—and requires all research staff and DCM employees to follow specified safety procedures. Pregnant individuals are advised to consult their physician before handling these agents.

Body

 

Title

University of North Carolina at Chapel Hill Standard Operating Procedure for Use of Antibiotics in Animals

EHS SOP #: 015

Version No.: 1.0 

Supercedes: N/A

Introduction

Purpose

This document establishes procedures for the safe handling and use of antibiotics in University Division of Comparative Medicine (DCM) facilities and research laboratories. Common antibiotics include doxycycline, neomycin, ampicillin, tetracycline, bleomycin, and rapamycin. Some classes of antibiotics are known or suspected reproductive toxins including quinolones, tetracyclines, sulfonamides, and macrolides. Pregnant individuals should consult their primary care physician before handling.

Scope

This Standard Operating Procedure applies to all research staff and DCM employees when working with these c agents in DCM facilities.

Standard Operating Procedure

Requirements

1. Administrative Controls

  • The Institutional Animal Care and Use Committee (IACUC) “Use of Chemical Hazards in Animals” form is not required for submission for use of the antibiotics listed in this SOP.
  • Anyone who handles antibiotics or works with animals to administer these agents are required to review this SOP and the attached Safety Data Sheet (SDS) prior to work.
  • Antibiotic use in animals must follow the Division of Comparative Medicine (DCM) SOP - Handling Cages Dosed with Chemical Hazards.

2. Engineering Controls

  • Procedures that involve handling and dissolving solid forms of antibiotics must be conducted under exhaust ventilation in a chemical fume hood or ducted Biological Safety Cabinet (BSC).

3. Personal Protective Equipment (PPE)

  • For handling, dissolving, or preparing mixtures of antibiotics, nitrile gloves, a lab coat, and safety glasses are required.
  • When in DCM facilities, PPE use must be consistent with facility policy.

4. Waste Disposal

  • Unused solutions and contaminated solid waste will be disposed of as hazardous material through EHS.
  • Excess water and excess or expired feed treated with the agent should be collected and disposed of as hazardous material through EHS.

5. Accidents or Injuries

  • Animal bites do not pose any increased risk. As per standard policy, report to supervisor.
  • If an antibiotic solution or material is splashed on an individual or in eyes flush for 15 minutes with copious quantities of water and contact the University Employee Occupational Health Clinic (919-966-9119).

6. Spill Procedures

  • Do not attempt to clean-up if you feel unsure of your ability to do so or if you perceive the risk to be greater than normal laboratory operations.
  • If a small spill occurs, absorb with absorbent pads. Double bag contaminated solid waste and submit as a hazardous material through EHS.
  • If a large spill occurs notify others in the area and evacuate room immediately. Contact EHS (919-962-5507) during working hours and 911 if after hours.

Contact Information

Primary Contact

Office: Environment, Health & Safety

Address: 1120 Estes Drive, Campus Box #1650, Chapel Hill, NC 27599-1650

Phone: 919-962-5507

Details

Details

Article ID: 159393
Created
Thu 11/20/25 3:47 PM
Modified
Fri 2/27/26 4:02 PM
Responsible Unit
School, Department, or other organizational unit issuing this document.
Environment, Health and Safety
Issuing Officer
Name of the document Issuing Officer. This is the individual whose organizational authority covers the policy scope and who is primarily responsible for the policy.
Issuing Officer Title
Title of the person who is primarily responsible for issuing this policy.
Executive Director, Department of Environment, Health & Safety
Next Review
Date on which the next document review is due.
02/27/2027 12:00 AM
Last Review
Date on which the most recent document review was completed.
02/27/2026 12:00 AM
Last Revised
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02/27/2026 12:00 AM
Effective Date
If the date on which this document became/becomes enforceable differs from the Origination or Last Revision, this attribute reflects the date on which it is/was enforcable.
02/27/2026 12:00 AM
Origination
Date on which the original version of this document was first made official.
02/27/2026 12:00 AM
Flesch-Kincaid Reading Level
14.0